Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: FATP1-mediated fatty acid uptake in renal tubular cells as a countermeasure for hypothermia

doi: 10.1007/s00109-025-02525-0

Figure Lengend Snippet: Effect of low temperature on cultured mouse proximal tubular cells. A Volcano plot representing the gene expression variation when mouse renal proximal tubular epithelial cells (MRPTEpiCs) were incubated at low temperature (28 °C, n = 5) compared with standard culture temperature (37 °C, n = 5). B Normalized enrichment score analyzed by gene set enrichment analysis (GSEA; Gene Ontology biological process). C Representative GSEA results. D Slc27a1 mRNA expression levels in MRPTEpiCs cultured with standard and low temperatures, RNA sequencing data (left) and qRT-PCR data (right) ( n = 5, respectively). E FATP1 protein expression levels in MRPTEpiCs analyzed by immunofluorescence staining. Scale bars: 10 μm. F Western blot analysis of FATP1 protein expression in MRPTEpiCs cultured at standard and low temperatures ( n = 5, respectively). G Analysis of lipid accumulation in MRPTEpiCs cultured at standard and low temperatures. BODYPI (green) and DAPI (blue). Scale bars: 10 μm. H Quantitative analysis of fluorescence intensity representing accumulated lipids in MRPTEpiCs cultured at standard and low temperatures ( n = 16 each). I Oxygen consumption rate (OCR) determined for MRPTEpiCs incubated under standard and low temperature conditions by Seahorse analysis. Oligomycin, FCCP (carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone) and rotenone/antimycin were added at the indicated time points ( n = 3, respectively). Data were analyzed by one-way ANOVA followed by the Mann–Whitney U test or Turkey-Kramer method. All data are represented as the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, **** P < 0.0001. Cont: control group; Hyp: hypothermia group

Article Snippet: Mouse renal proximal tubular epithelial cells (MRPTEpiCs) were purchased from Innoprot (Bizkaia, Spain).

Techniques: Cell Culture, Gene Expression, Incubation, Expressing, RNA Sequencing, Quantitative RT-PCR, Immunofluorescence, Staining, Western Blot, Fluorescence, MANN-WHITNEY, Control

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: FATP1-mediated fatty acid uptake in renal tubular cells as a countermeasure for hypothermia

doi: 10.1007/s00109-025-02525-0

Figure Lengend Snippet: Effect of FATP1 suppression or renal injury on hypothermia. A Schematic depiction of the experimental design. RT: kept at room temperature; Hyp: kept at 4 °C; FATP1-in-1: mice treated with 20 mg/kg FATP1-in-1. B Changes in body temperature in FATP1-inhibited model mice ( n = 5 each). C FFA and TG levels in renal tissue extracts from the model mice ( n = 5 each). D Hematoxylin and eosin (H&E) staining and immunohistochemical staining of cleaved-PARP in the model mice. Scale bars: 50 μm. E The number of cleaved-PARP-positive cells in renal proximal tubular cells. The average of five fields of view at high magnification (× 400). F Cytotoxicity LDH assay and SRB assay in mouse renal proximal tubular epithelial cells (MRPTEpiCs) incubated under standard (37 °C) and low-temperature (28 °C) conditions with palmitic acid (PA) and FATP1-inhibitor ( n = 6). Data were analyzed by the Mann–Whitney U test or one-way ANOVA followed by the Turkey-Kramer method. All data are represented as the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Cont: control group; Hyp: hypothermia group; FATP1-in-1 RT: FATP1 inhibitor room temperature group; FATP1-in-1 Hyp: FATP1 inhibitor hypothermia group

Article Snippet: Mouse renal proximal tubular epithelial cells (MRPTEpiCs) were purchased from Innoprot (Bizkaia, Spain).

Techniques: Staining, Immunohistochemical staining, Lactate Dehydrogenase Assay, Sulforhodamine B Assay, Incubation, MANN-WHITNEY, Control